DNA Isolation Kit, Serum, BioAssay™

RT

Molecular Biology Grade

Description:
The Serum DNA Isolation Kit is designed for the purification of genomic DNA fragment from serum, plasma, and bio fluid in a spin column format. No phenol-chloroform extraction, no Protease and no precipitation steps are involved. The sample addition and washing steps can be performed using compatible vacuum manifold, while the final elution of the DNA product are performed using a table- top centrifuge.
The samples are first treated in the binding buffer that contains the denaturant guanidine HCl and Triton X100. Ethanol is then added to the samples, which are then added to the filter spin columns. This step facilitates the binding of DNA to the filter matrix. Under these conditions the DNA binds to the membrane while other contaminants are washed through. The columns are then washed to further remove protein, buffer components and other contaminants using two ethanol-containing wash buffers and the final genomic DNA product is eluted in TE. The final DNA product can be used directly for quantitative PCR and other downstream applications.

Quality Control:
Each lot of Serum DNA isolation Kits is tested against predetermined specifications to ensure consistent product quality.

Item
Amount
Storage

1. Lysis Binding Buffer
21ml
RT

2. Wash Buffer 1
26ml
RT

3. Wash Buffer 2
12ml
RT

4. TE
10ml
RT

5. spin column set
100 units
RT