CXCL1 BioAssay™ ELISA Kit, Human

Kits and Assays / Kits-ELISA (Chemokines and Chemokine Receptors), BioAssay™

4°C/-20°C

Sandwich ELISA quantitative detection kit for Human CXCL1 in cell culture supernates, serum and plasma (heparin, EDTA, citrate).

Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.5 The standard product used in this kit is recombinant human CXCL1, consisting of 77 amino acids with the molecular mass of 8kD.

Assay Range:
15.6pg/ml-1000pg/ml

Sensitivity:
< 1 pg/ml

Crossreactivity:
There is no detectable cross-reactivity with other relevant proteins.

Uniprot ID:
P09341

Entrez Gene ID:
2919

Kit Components:
96-well plate pre-coated with anti-CXCL1, Human
Lyophilized recombinant CXCL1, Human standard, 10ng/tube, 2Ea
USB Cat No Kit Component
143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)
143697 Sample diluent buffer, 30ml
143698 Antibody diluent buffer, 12ml
143699 ABC diluent buffer, 12ml
143700 TMB color developing agent, 10ml
143701 TMB stop solution, 10ml

Storage and Handling:
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.