Matrix Metalloproteinase 9 (MMP9) BioAssay™ ELISA Kit (Bovine)

Blue Ice

38220000

The Matrix Metalloproteinase 9 (MMP9) BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MMP9 in bovine serum, plasma, tissue homogenates and other biological fluids.

Detection Range:
0.312-20ng/ml

Sensitivity:
0.123ng/ml

Precision:
Intra-Assay %CV: <10%
Inter-Assay %CV: <12%

Kit Components:
*152807A: Microtiter Strips, 1x96 wells Pre-coated, ready to use
*152807B: Standard, 2x1 vial
152807C: Standard Diluent, 20ml
*152807D: Detection Reagent A (green), 1x120ul
*152807E: Detection Reagent B (red), 1x120ul
152807F: Assay Diluent A, 1x12ml
152807G: Assay Diluent B, 1x12ml
152807H: TMB Substrate, 1x9ml
*152807J: Positive Control, Lyophilized, 2.42ng/ml, 1x1vial
152807K: Stop Solution, 1x6ml
152807L: Wash Buffer, 30X, 1x20ml

Precaution:
The Stop Solution (152807K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability:
Store *152807A, *152807B, *152807D, *152807E and *152807J at -20°C. After reconstitution of *152807J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 152807J: Positive Control:

Intended Use:
The control vial is from a bovine serum sample known to contain BNP. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics:
The control vial contains bovine MMP9 in bovine serum (lyophilized powder). Note: The porcine source material used in this product is from healthy porcine of SPF standards (specific pathogen-free animals).

Expected Values:
2.42ng/ml

Usage:
Dissolve with 150ul standard diluent and let it stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens.
Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.

Assay Procedure Summary:
1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substmousee. Incubate 10-20 minutes at 37°C.
8. Add 50ul Stop Solution. Read at 450nm immediately.