MMP-3 total BioAssay™ ELISA Kit (Human) (Stromelysin-1, SL-1, Matrix metalloproteinase-3, MMP 3, Transin-1, MMP3, STMY1)

Kits and Assays / Kits-ELISA (Metalloprotease), BioAssay™

4°C/-20°C

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Sample Type:
Cell culture supernatants, serum, plasma, and tissue

Intended Use:
The Human MMP-3 total BioAssay™ ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human MMP-3 total concentrations in cell culture supernatants, serum, plasma, and tissue.

Sensitivity:
15pg/ml

Range:
62.5-4000pg/ml

Specificity:
Recognizes human MMP-3 total.

Sample Volume:
100ul/well

Test Principle:
This assay employs the Sandwich immunoassay technique. An anti-h MMP-3 monoclonal coating antibody is adsorbed onto microwells. MMP-3 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h MMP-3 antibody is added and binds to MMP-3 captured by the first antibody. Following incubation unbound Biotinylated anti-h MMP-3 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h MMP-3 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of MMP-3 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven MMP-3 standard dilutions and MMP-3 sample concentration determined.

Kit Components:
Aluminum pouches with a Microwell Plate: 8x6
Standard: 1xVial
Biotinylated antibody: 1xVial
Streptavidin-HRP: 1xVial
Assay Buffer (25ml/Vial): 1xVial
Wash Buffer Concentrate 20x (30ml/Vial): 1xVial
TMB Substrate Solution: 1x6ml
Stop Solution (12ml/Vial): 1xVial
Plate Covers- Adhesive strips: 2xstrips
Instruction: 1x

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.