genesig Real-Time PCR detection kit for H1N1 influenza, Advanced

Kits and Assays / Kits-PCR, genesig™

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genesig Advanced kit:

This genesig® Complete qPCR detection kit targets the Haemagglutinin (HA) gene and Matrix protein (M1) gene from Influenza A virus subtype H1N1 which is commonly known as H1N1. H1N1 is an RNA virus which primarily infects humans and swine. Infection can lead to Influenza (Flu).

Product features:
•Exceptional value for money
•Rapid detection of all clinically relevant subtypes
•Positive copy no. standard curve for quantification
•Highly specific detection profile
•High priming efficiency
•Broad dynamic detection range (>6 logs)
•Sensitive to < 100 copies of target
•Accurate controls to confirm findings

Instrumentation Requirements:
Designed for use in other instruments. For use in genesig® q16, look for the “Easy” nomenclature.

genesig® kits are sold for research use only and are not licensed for diagnostic procedures

ABOUT THE genesig® KITS:
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative Real-Time PCR kits for the detection and simultaneous quantification of numerous significant pathogens. A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented Real-Time PCR expertise in design and validation ensure the best possible kit. 

Details of the target and priming specificity are included in the individual handbooks above. Packaged, optimized and ready to use.

Specificity:
The kit is designed for the in vitro quantification of Influenza A virus subtype H1N1 genomes and to have a broad detection profile. Specifically, the primers will detect over 95% of sequences available on the GISAID EpiFlu at the time of last review. The Swine_H1 primer and probe set is designed to detect all H1N1 sequences from the H1N1 lineage but may cross react with a subset of H1N2 sequences.

Dynamic Range of Test:
Under optimal PCR conditions the kit can achieve priming efficiencies between 90-110% and detect less than 100 copies of target template. If running a positive control standard curve for a quantitative result, and an efficiency of between 90% to 110% is not achieved, then the run should be repeated with a freshly prepared standard curve

ADVANCED KIT CONTENT:
•Advanced kit contents:
•Primer and probe mix (150 reactions)
•Reverse Transcription, target specific primers (RNA genome viruses only)
•Copy number standard curve (sufficient for multiple standard curves)
•Internal extraction control - Read through VIC channel* (150 tests)
•Endogenous control (150 tests)
•RNAse/DNAse free water

DNA/RNA Extraction:
genesig® Easy DNA/RNA extraction kits are a simple to use, low cost and powerful extraction technology suitable for virtually all sample types.