Tissue, Genomic DNA, Human Tumor Cell Line, Hela (Cervix Adenocarcinoma), BioGenomics™

Biologicals / Biologicals-Tissues-Genomic DNA, Human

-20°C

Supplied as a liquid in 10mM Tris, pH 8.0, 1mM EDTA.

Genomic DNAs are isolated from over 100 different human and fetal normal tissues, human diseased and tumor tissues, mouse, rat, monkey, and plant tissues. Our genomic DNAs are provided ready to use for SNP analysis, Southern Blotting, and PCR. United States Biological offers almost any genomic DNAs from human, mouse, rat, monkey, and plant tissues; no longer will you need to acquire tissues and isolate genomic DNA by yourself.

Applications:
Suitable for use in SNP analysis, Southern Blot, PCR, Genomic DNA library construction and Profiling study in gene expression. Other applictions not tested.

Donor Information:
Human Tumor Cell Line (HeLa)

Quality Control:
1. Quality and purity of genomic DNA was tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0 (detected in 10mM TE, pH 8.0).
2. RNAse treatment to ensure there is no RNA contamination.
3. Genomic DNA was successfully digested by Hind III.
4. B-Actin expression was tested by PCR amplification for Genomic DNA.
5. GAPDH expression was tested by Real Time PCR for 96 well tumor genomic DNA plate.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.