BTNL2: Fc, Recombinant, Mouse, aa26-457 (Butyrophilin-like Protein 2)

Molecular Biology / MB-Enzymes

-20°C

Supplied as a lyophilized powder in PBS. Reconstitute with 1ml sterile PBS.

Butyrophilin-like 2 (BTNL2) is a butyrophilin family member with homology to the B7 costimulatory molecules, polymorphisms of which have been recently associated through genetic analysis; to sporadic inclusion body myositis and sarcoidosis. BTNL2 mRNA is highly expressed in lymphoid tissues as well as in intestine. BTNL2-Ig fusion protein was shown to recognize a putative receptor whose expression on B and T cells was significantly enhanced after activation. It inhibited T cell proliferation and TCR activation of NFAT, NF-kappaB and AP-1 signaling pathways. BTNL2 is the first member of the butyrophilin family that regulates T cell activation, which has implications in immune diseases and immunotherapy.

Source:
Recombinant protein corresponding to aa26-457 from mouse BTNL2, fused to the N-terminus of the Fc region of mouse IgG2a, expressed in CHO cells.

Biological Activity:
A butyrophilin-like molecule that functions to inhibit T cell activation.

Endotoxin:
<0.06EU/ug protein (LAL test; Lonza)

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.