Protease Inhibitor Cocktail, Set VII

Molecular Biology / MB-Protease Inhibitors

-20°C

Supplied as a liquid in 1ml DMSO.

A cocktail of five protease inhibitors with broad specificity for the inhibition of cysteine, serine, aspartic, and thermolysin-like proteases and aminopeptidases. This cocktail is recommended for purification of proteins containing His-tag sequences. Each vial contains 100mM AEBSF, HCl, 5mM Bestatin, 1.5mM E-64, 2mM Pepstatin A, and 200M of Phosphoramidon, Disodium Salt. One ml is recommended for the inhibition of proteases in 10g of cells. Supplied with a data sheet. Note: 1set=5x1ml.

Kit Components:
AEBSF, Hydrochloride: Molecular weight: 239.5, 1X100mM. Target Protease: Serine Proteases
Bestatin: Molecular weight: 308.4, 1X5mM. Target Protease: Aminopeptidase B and Leucein Aminopeptidase
E-64 Protease Inhibitor: Molecular weight: 357.4, 1X1.5mM. Target Protease: Cysteine Proteases
Pepstatin A: Molecular weight: 685.9, 1X2mM. Target Protease: Aspartic Proteases
Phosphoramidon: Molecular weight: 587.5, 1X0.2mM. Target Protease: Metalloendopeptidases

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.