EMAP II, Recombinant, Human (AIMP1, EMAP2, EMAP-2, EMAPII, SCYE1, Multisynthetase Complex Auxiliary Component p43, Endothelial Monocyte-activating Polypeptide 2, EMAP-II p43)

Blue Ice

18, 3

Supplied as a lyophilized powder from 20mM sodium phosphate, pH 7.5, 130mM sodium chloride. Reconstitute with sterile ddH2O to a concentration ≥0.1mg/ml.

Endothelial-Monocyte Activating Polypeptide-II (EMAP-II) is a tumor derived cytokine that is an anti-angiogenic factor in tumor vascular development, and strongly inhibits tumor growth.

Recombinant protein corresponding to 166aa residues from human EMAP II, expressed in E. coli.

Biological Activity:
Determined by its ability to induce apoptosis in MCF-7 cells using a concentration range of 20-30ng/ml.

Amino Acid Sequence:
SKPIDVSRLD LRIGCIITAR KHPDADSLYV EEVDVGEIAP RTVVSGLVNH VPLEQM QNRM VILLCNLKPA KMRGVLSQAM VMCASSPEKI EILAPPNGSV PGDRITFDAF PGEPDKELNP KKKIWEQIQP DLHTNDECVA TYKGVPFEVK GKGVCRAQTM SNSGIK

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.