MECP2, Recombinant, Human, aa78-162, GST-Tag (Methyl CpG Binding Protein 2, Rett Syndrome)

Dry Ice

36

Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 124mM sodium chloride, 2.2mM potassium chloride, 18mM glutathione, 3mM DTT, 10% glycerol.

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq, Jul 2009].

Source:
Recombinant protein corresponding to aa78-162 from human MECP2, fused to GST-tag at N-terminal, expressed in E. coli.

Molecular Weight:
~36kD

Applications:
Suitable for use in the study of DNA binding, screening inhibitors, and selectivity profiling. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.