Interleukin-36 receptor antagonist, active (IL36RN), Recombinant, Human

Blue Ice

16, 8

Lyophilized from 0.2um filtered PBS, pH 7.4

Inhibits the activity of interleukin-36 (IL36A, IL36B and IL36G) by binding to receptor IL1RL2 and preventing its association with the coreceptor IL1RAP for signaling. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor. Proposed to play a role in skin inflammation. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus. May activate an anti-inflammatory signaling pathway by recruiting SIGIRR. {ECO:0000269|PubMed:11466363, ECO:0000269|PubMed:21965679, ECO:0000269|PubMed:23147407}.

Biological Activity:
Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-36 beta induced IL-8 secretion by human preadipocytes is less than 1.0ug/ml, corresponding to a specific activity of ＞ 1000 IU/mg in the presence of 20ng/ml of recombinant human IL-36 beta.

Sequence:
VLSGALCFRM KDSALKVLYL HNNQLLAGGL HAGKVIKGEE ISVVPNRWLD ASLSPVILGV QGGSQCLSCG VGQEPTLTLE PVNIMELYLG AKESKSFTFY RRDMGLTSSF ESAAYPGWFL CTVPEADQPV RLTQLPENGG WNAPITDFYF QQCD

Molecular Weight:
16.8kD

PubMed ID:
10625660; 10512743; 11093146; 11466363; 10860666; 11991722; 12975309; 14702039; 15815621; 15489334; 21965679; 23147407; 21839423; 21848462; 22903787

Gene Name:
IL36RN, FIL1D, IL1F5, IL1HY1, IL1L1, IL1RP3, UNQ1896/PRO4342

Swiss Prot:
Q9UBH0

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.