Griffonia simplicifolia GS I (Bandeiraea simplicifolia) (Rhodamine)

Molecular Biology / MB-Lectin Griffonia simplicifolia

-20°C

Supplied as a liquid in 10mM HEPES, 0.15M sodium chloride, pH 7.5, 0.08% sodium azide, 0.1mM Ca++. Labeled with Rhodamine.

GSL I is a family of glycoproteins with molecular weights of approximately 115, 000. There are two types of subunits, termed “A” and “B”, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The “A”-rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for a-N-acetylgalactosamine residues, while the “B”-rich lectin preferentially agglutinates blood group B cells and is specific for a-galactose residues. Our GSL I is a mixture of the five isolectins. Binding appears to require divalent cations such as calcium and magnesium. GSL I has been reported to bind several glycoproteins including laminin. Rhodamine labeled Griffonia Simplicifolia Lectin I is produced by using the highest quality tetramethylrhodamine isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Rhodamine labeled Griffonia Simplicifolia Lectin I has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin. Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions perserved with sodium azide.

Applications:
Suitable for use in FLISA. Other applications not tested.

Recommended Dilution:
FLISA: 5-20ug/ml
Optimal dilutions to be determined by the researcher.

Absorption:
5450-555nm

Emission:
570-580nm

F/P (molar):
6.6

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.