p70 S6 Kinase, active, Recombinant, Human (p70 S6K)

Molecular Biology / MB-Protein Kinases

-70°C

Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

p70S6K is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues (1). p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6K activity undergoes changes in the cell cycle and increases 20-fold in G1 cells released from G0 (2). p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as T389, a site phosphorylated by phosphoinositide-dependent kinase 1 (PDK-1).

Recombinant full-length human p70 S6 Kinase (p70S6K) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Accession Number: NM_003161.

Molecular Mass:
~76kD.

Activity:
The specific activity of p70S6K was determined to be 76 nmol/min/mg using a synthetic peptide substrate (CKRRRLASLR) (see activity assay protocol).

Storage and Stability:
This product is stable at ≤ -70° C for at least 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.