POGLUT1, aa1-388, Recombinant, Human (Protein O-glucosyltransferase 1, CAP10-like 46kD Protein, hCLP46, KTEL Motif-containing Protein 1, Myelodysplastic Syndromes Relative Protein, C3orf9, CLP46, KTELC1, MDSRP, MDS010, UNQ490/PRO1006)

Blue Ice

44

Supplied as a liquid in Tris, sodium chloride.

O-Glucosyltransferase1 (POGLUT1) is a homologue of Rumi from Drosophila, an endoplasmic reticulum (ER)-retaining glucosyltransferase, that adds glucose to serine residues within the consensus sequence of C1-X-S-X-P-C2 in Notch EGF repeats, thereby regulating cell-fate decisions (1). It is also known as CAP10-like protein (2) and KTELC1 due to the highly conserved CAP10 domain and the presence of an ER-retaining motif, KTEL, at the C-terminus. The human gene is reported to be involved in the pathogenesis of both acute myelogenous and T-acute lymphoblastic leukemias (3). Recently, POGLUT1 has been demonstrated in a phosphatae- coupled glycosyltransferase assay to have hydrolase activity on UDP-Glc (4).

Source:
Recombinant corresponding to aa1-388 from human POGLUT, fused with 6-His tag at C-terminal, expressed in CHO cells.

Molecular Weight:
~44kD

Endotoxin Level:
≤1EU/1ug (LAL)

Biological Activity:
Recombinant corresponding to aa1-388 from human POGLUT CHO cell line, with a C-terminal 6-His tag expressed in CHO cells.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

POGLUT1, aa1-388, Recombinant, Human (Protein O-glucosyltransferase 1, CAP10-like 46kD Protein, hCLP46, KTEL Motif-containing Protein 1, Myelodysplastic Syndromes Relative Protein, C3orf9, CLP46, KTELC1, MDSRP, MDS010, UNQ490/PRO1006)