Phaseolus vulgaris L (PHA-L, Red Kidney Bean) (Agarose)

Blue Ice

128

Supplied as a supension in 0.01 PBS, pH 7.2-7.4, 0.05% sodium azide covalently bound to 4% agarose.

Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms.

Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.

Protein Concentration:
4-5mg PHA-E/ml settled beads.

Bead Size:
50-250 microns

Spacer and Bead Linkage:
Imidoester

Spacer:
None

Carbohydrate for Elusion:
0.1M Glycine pH 3.5-4.0. Simple sugars are not effective for elution.

Quality Control:
SDS-PAGE

Storage and Stability:
For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.