Phosphatase, Alkaline, Calf Intestine, Solution, 4000U/mg Protein

Blue Ice

110150

Supplied as liquid, crystalline suspension in 70% ammonium sulfate. Free of endonuclease, exonuclease and RNase activities. No preservatives added.

Native calf alkaline phosphatase.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
May be diluted in sterile ddH2O or in dilute buffer. Optimal dilutions to be determined by the researcher.

Activity:
4000U/mg protein. 1 unit liberates 1umole of p-nitrophenol per minute at 37ºC and pH 9.8 in DEA buffer. Activity is determined by measuring the increase in the optical density at 405nm resulting from the hydrolysis of p-notrophenyl phosphate.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Phosphatase, Alkaline, Intestine, Bovine (Alkaline Phosphatase Intestinal, ALPI, Alkaline Phosphomonoesterase, Glycerophosphatase, Intestinal Alkaline Phosphatase, IAP, Intestinal Alkaline Phosphatase V, IAP-V, Kasahara Isozyme)