Plasminogen Activator Inhibitor Type 1 mutant, vitronectin binding (PAI-1)

Molecular Biology / MB-Vitronectin

-70°C

Highly Purified

Plasminogen Activator Inhibitor 1 (PAI-1) is a glycoprotein found in plasma that is implicated in the processes of angiogenesis, hemostasis, tumor metastasis, fibrionlysis, ovulation, embryonic development and inflammation (1). The elevation of PAI-1 has shown to correlate with the prognosis of many cancers including breast cancer (2, 3, 4). PAI-1 is activated by the glycoprotein vitronectin. Vitronectin binds to PAI-1 and modulates fibrinolysis and cell migration (5). This binding has proven to be an important inhibitor of the activated form of protein C (APC) (6).

Physical Appearance:
Colorless frozen solution

Solubility:
>2mg/ml and <5mg/ml

Buffer Composition:
0.05M sodium phosphate; 0.1M sodium chloride; 1mM EDTA; pH 6.6

Purity:
>98% pure by SDS PAGE 95 (+/-) 5% active by uPA titration.

Absorbance (280nm): 3.6 E0.1% = 1.0
Concentration = 3.6mg/ml
Active Concentration = 3.4mg/ml

Kinetic Data:
Second order rate constants for inhibition of:
UPA = 6.3 X 106 M^-1s^-1
tPA = 5.7 X 106 M^-1s^-1

Storage and Stability:
Store frozen at -70°C – stability >1 year.