Protein G, Native (FITC)

Blue Ice

33

Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC) (MW 390D).

Native Protein G is a surface receptor from Streptococcus sp. which binds the Fc portion of immunoglobulins, binding 2 molecules of IgG.

Applications:
Suitable for use in Immunomicroscopy and FLISA. Other applications not tested.

Recommended Dilutions:
Immunomicroscopy: 1:500-1:2500
Optimal dilutions to be determined by the researcher.

Reconstitution:
Use 1ml of ddH2O. For extended storage reconstitute product with sterile 50% glycerol, ddH2O instead of water and then aliquot contents and freeze at -20°C or lower.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.