Secretory Leukocyte Protease Inhibitor Recombinant, Human, aa26-132 (SLPI, ALK1, ALP, BLPI, HUSI, HUSI-I, WAP4, WFDC4 Four-disulfide core domain 4, Antileukoproteinase, Mucus proteinase inhibitor, Seminal proteinase inhibitor)

Molecular Biology / MB-Protease Inhibitors

-20°C

Supplied as a lyophilized powder from a sterile-filtered PBS, pH 7.4. Reconstitute with 200ul sterile PBS.

Human Secretory Leukocyte Protease Inhibitor SLPI is a protein found in various secretory fluids, including parotid secretions, bronchial, nasal and cervical mucus, and seminal fluid. The protein is a potent inhibitor of a variety of proteolytic enzymes, including the neutrophil proteases, elastase and cathepsin G and the pancreatic proteases chymotrypsin and trypsin. SLPI is an important component of the antiprotease defense of tissues bathed by secretory fluids and could be useful as a therapeutic agent for treatment of the proteolytic damage of tissues seen in degenerative and inflammatory diseases.

Recombinant protein corresponding to Ser26-Ala132 from human SLPI, expressed in E. coli.

Predicted Molecular Weight:
~12kD

Activity:
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca­RPKPVE­Nval­WRK(Dnp)­NH2. The IC50 value is ≤4.0nM, under the described conditions. See Activity Assay Protocol.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Secretory Leukocyte Protease Inhibitor Recombinant, Human, aa26-132 (SLPI, ALK1, ALP, BLPI, HUSI, HUSI-I, WAP4, WFDC4 Four-disulfide core domain 4, Antileukoproteinase, Mucus proteinase inhibitor, Seminal proteinase inhibitor)