Serum, Canine

Biologicals / Biologicals-Serum, Plasma-Animal

-20°C

Supplied as a lyophilized powder. No preservative added. Reconstitute with 1ml sterile ddH2O, mix gently. Allow to stand 5-10 minutes at RT to reach equilibrium.

Description:
Pooled normal canine serum

Applications:
Suitable for use as a qualitative serum with an optimized protein content to study the normal protein composition of canine serum. To identify or quantitate a normal serum protein component using a variety of immunodiffusion techniques including immunoelectrophoresis, single and double radial immunodiffusion and electroimmunodiffusion. As a reference serum in nephelometry and other automated precipitation techniques. As a blocking agent or as a negative control in nonprecipitating antibody-binding assays e.g. in serodiagnostic immunofluorescence and immunoenzyme tests. This normal reference serum does not contain assigned values for individual proteins but can be used as an internal relative standard for quantitative protein assays such as double radial immunodiffusion (Mancini, Fahey), ELISA,
Western Blot and electroimmunodiffusion (Laurell), expressing the results obtained with the serum sample under investigation as a percentage of the proteinconcentration in the normal reference serum.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.