Mouse Negative Control, Ascites

Biologicals / Biologicals-Serum, Plasma-Animal

-20°C

Supplied as a lyophilized powder. No preservatives added. Reconstitute with 1ml sterile ddH2O. Dilute further with PBS.

Mouse ascites obtained from mice bearing different hybridomas, delipidated, heat-inactivated, pooled and depleted of all immunoglobulins.

Applications:
Suitable for use as a negative control for ELISA, Indirect Immunofluorescence and Western Blot, replacing the specific ascites in the test arrangement. Other applications have not been tested.

Recommended Dilutions:
The control ascites is used in a dilution comparable to that of the specific ascites.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.