Mouse L3 Array, Membrane

Multiplexed Protein Detection
Detection of Relative Protein Expression
Detecting Patterns of Cytokine Expression
Biomarker Screening
Identifying Key Factors
Confirming a Biological Process
Cross-species testing

PTEN, AKR1A1, ATOX1, CNPY2, EIF3D, NSFL1C, PLBD2, PPP1CC, PSME1, PSME2, TPPP3, TTF1, COL8A2, CPA3, CTNNAL1, DIAPH1, KCNAB3, SHOX, AK1, Dystrophin, Nectin-4, Cytochrome b5, NQO2, Nucleobindin 1, OIT3, Orosomucoid 2, OSBP1, OSCAR, Oxytocin-neurophysin 1, p16 ARC, p23, p39, P4HB, p73, PACS1, PARVB, PCBP1, PCBP2, PCCA, PCK2, PCMT1, PCNA, PCPE-1, PDE1B, PDIA6, PDLIM1, PDZD2, PEBP4, PEPD, Perilipin-1, Periostin, Peroxiredoxin 2, Peroxiredoxin 3, PGAM1, PGAM2, PGK-1, PGM1, PGRPL, pIgR, PIN, PISD, PLA2G1B, Plastin L, Plectin, Plexin B1, Plexin B2, PLOD1, PLOD2, Plxdc2, PNP, POR, PPOX, PPP2R1B, PPP2R4, PRCP, PRDM13, PREP, PRG2, Prion protein PrP, Profilin 1, Prosaposin, Prostaglandin D Synthase, Protein Z, PRSS23, PSMA1, PSMA2, PSMA4, PSMB5, PSMC3, PSMD1, PSMD9, PTK 7, PTMA, PTPRZ, PZP, QDPR, QPRT, Quiescin Q6, Rab7a, Ran, RanGAP1, Rbm15, RCL, Reg3A, RHOC, Ribonuclease A, Ribonuclease T2, RNASE4, RPL10, RPL10A, RPL11, RPL12, RPL14, RPL17, RPL22, RPL7A, RPLP0, RPS10, RPS11, RPS12, RPS19, RPS2, RPS20, RPS23, RPS25, RPS3, RPS5, RREB1, RSU1, S100 A1, S100 A7, SBP-1, SDNSF, SDPR, Secretogranin V, Semaphorin 6B, Semaphorin 7A, Serpin A11, Serpin A7, Serpin B1, Serpin B4, Serpin B8, Serpin E2, Serpin F2, SerRS, SET, SEZ6L2, SF20, SHANK1, SHC1, SHMT1, SHP-1, SIM2, Six3, SLITRK1, SLURP1, SMC4, SMPD4, SOD1, SOD2, Somatostatin, SORD, SorLA, SOX4, SP-D, SPEN, STAT3, Stathmin 1, STI1, STOM, SUCLG1, SUMO3, Symplekin, Syntaxin 7, TAB182, Talin1, TAX1BP3, TBCA, TCEB2, Tcf20, TCP1 eta, TGM3, Thioredoxin-1, TOB2, TOP2B, TPM4, Transthyretin, TRAP1, TRAP220, TRF 2, Tropomyosin 3, TRP-1, TRPS1, Trypsinogeb-2, TSR2, TUBA6, TWF2, TXNDC4, TXNDC5, TXNRD2, UBE2D3, Ube2L3, UCH-L1, UFM 1, UGGT, UNC45A, UNC5H4, UPB1, UQCRB, UQCRH, URB, URB2, UROC1, UROD, URP2, USP14, USP5, Utrophin, VAP-A, VCP, VDAC1, VNN1, VSIG4, WDR1, WISP2, WNK2, YB1, YY1, ZBTB4, ZC3H4-N-t, ZC3H8, Zyxin, MRC2, SOX5, SPTBN2, STXBP2, SVEP1, AHCY, Annexin A3, ASS1, CSRP3, CORO1B, ENDOD1, Histone H1.0, Protocadherin-12, SNRPD1, ANKRD9, Dematin, PDAP1, RHOG, TOMM70A, TPD52L2, WDR44, BBS1, MTHFD1, MVD, NAGPA, NFATC4, NNT, QARS, RPL23A, SMAD6, ZBTB11, Serpin B6, Src, Serpin A10, Tenascin C, GLI-2, gp340, PEBP1, PFAS, PHGDH, Prolargin, S100A11, S100A9, TXNDC15, USP2, VILIP3, AARE, ACAT1, acyl-CoA Thioesterase 2, ALDH2, alpha 5 D, AP180, AP3S2, APLP2, ASPM, ATPG, AUTS2, BAI2, BarX1, BE2I / UBC9, BLM, BOLA2, C10orf58, CACNA1H, Calpain 2, CaMK2, CaMK2D, CBL, CBR1, CCDC58, CCT6A, CHCHD3, Cingulin, CIT, CMG1, CNBP, Coilin, COLEC11, COPG2, CPI17 alpha, CrkRS, CRLF3, CTNND1, Cyclophilin F, DCAMKL1, DKC1, DLST, DMRT1, Ebf4, EBP50, ECHDC1, EHHADH, eIF4A2, eIF4GII, EYA2, Factor VIII, Filaggrin, FITM1, GARS, GCC2, GLOD4, GLUL, GMPR1, GOLGA3, GP2, GTF2F1, HA1, HARS, HIC1, HIP55, Histone H1.5, HIVEP2, hnRNP K, hnRNP R, HNRNPUL2, HNRPA3, HP1 g, Importin 7, Involucrin, ISLR, ITPR2, ITPR3, Laminin alpha 5, LDB3, LHPP, LIPG, MAP4K4, MICALL2, MON2, MPST, MSH3, MTA2, MUC5B, Myosin IIB, NACA1, NAV2, NPEPPS, NUP214, OAF, OTC, OTUB1, OTUD7A, PABP1, Pancreatic Lipase, PCAP, PCDH8, PDLIM3, PENK, perilipin 3, Periplakin, PFDN6, PFKL, PG-M, PGLS, Piccolo, PIK3C2B, PIP5K2 alpha, Plastin 3, PLD4, PMCA, POLD2, POLR2A, PPP1R9A, Proteasome 26S S2, PRRC2A, PRTN3, PSMA5, PSMA6, PSMB1, PSMB2, PSMB3, PSMB4, PSMB6, PSMB7, PSMD5, PTBP1, PTGR1, PTPRG, PTPRK, PTPRM, Rab1A, RanBP1, RAP1B, RECQ4, REV3L, RLF, Rnose2, RP1, RPL3, RPL32, RPL4, RPL7, RPLP2, RPS13, RPS14, RPS15A, RPS16, RPS18, RPS3A, RPS4X, RPS8, RPS9, RSF1, RUSC1, S E P T 7, SAA4, SC35, SCG, SCN3A, SCP2, SECISBP2, SERBP1, Serpin B3D, Serpin H1, SIRPB1, SIRPB1����, SLC4A1, SOD-3, Somatoliberin, Spectrin, SPG48, SPS2L, SPTLC1, SSC5D, Synemin, SYNPO2L, TARS, TCP1 delta, TCP1 theta, TCTP, TDIF2, Tenascin XB, TFF2, TIF1 alpha, TMEM103, TPR, TRIM14, TTC3, UBA1, UBE2N/Ubc13, UMP/CMP, UNC13 Homolog D, Uroguanylin, valyl tRNA, Vimentin, VPS4B, XPG, YN1 / Synapsin 1, ZAK, ZC3H18, ZNF295, ADAM-28, LEKTI / SPINK5, Uteroglobin / SCGB1A1, Coagulation Factor XIV / Protein C / F14, Proprotein Convertase 9 / PCSK9, SAA / Serum Amyloid A, Thimet Oligopeptidase / THOP1, OBCAM / OPCML, Osteoadherin / Osteomodulin, Oncostatin M R beta / IL-31 R beta, Peroxiredoxin-1 / PRDX1, Siglec-1 / CD169, Tripeptidyl-peptidase I / TPP1, Trypsin 3 / PRSS3, VAP-1 / AOC3, Transaldolase 1 / TALDO1, IGSF4A / SynCAM1, Apolipoprotein A-V / ApoA5

For best results, store Box 1 frozen at -20°C and Box 2 at 4°C upon arrival. Stored this way the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

500

Label-based

Semi-Quantitative

Ray Bio® L-Series Antibody Array Membranes
Labeling Reagent
Stop Solution
Blocking Buffer
Detection Buffer C
Detection Buffer D
Wash Buffer 1
Wash Buffer 2
HRP-Conjugated Streptavidin Concentrate
Plastic Sheets
Spin Columns
Labeling Buffer
Lysis Buffer
Plastic Incubation Tray

Purify Samples with Spin Column
Biotinylate Samples
Purify biotinylated samples using spin column
Block array membranes
Incubate with Samples
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

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