Chk2 (phospho Ser516) Polyclonal Antibody

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In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. Checkpoint kinase 2 encoded by CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, Checkpoint kinase 2 interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for CHEK2.

Synthesized peptide derived from human Chk2 around the phosphorylation site of S516

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Phospho-Chk2 (S516) Polyclonal Antibody detects endogenous levels of Chk2 protein only when phosphorylated at S516.