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Bovine Mucin-1 (MUC1) ELISA Kit

Bovine Mucin-1 (MUC1) ELISA Kit

ArtNr	ABK-KTE10240-96Tx50
Hersteller	Abbkine Scientific
Menge	96 T x 50
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Kategorie	Oncology By Cell Type Carcinoma Hematology Myeloma Cell Biology Cell Status ELISA Autophagy ELISA & Immunoassays ELISA Kits Immunoassay By Organ Breast Cancer Testicular & Prostate Cancer
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Cattle (Bovine)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	MUC1,RP11-263K19.2,CD227,EMA,H23AG,KL-6,MAM6,PEM,PEMT,PUM,DF3 antigen,H23 antigen,MUC-1/SEC,MUC-1/X,MUC1/ZD,breast carcinoma-associated antigen DF3,episialin,mucin 1,mucin 1,transmembrane,peanu
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Manufacturer - Applications	This Bovine Mucin-1 (MUC1) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC1 in samples. An antibody specific for MUC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMUC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Background	Mucin-1 (MUC1) encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. MUC1 is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. MUC1 is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. MUC1 is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Bovine Mucin-1 (MUC1) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine MUC1. No significant cross-reactivity or interference between Bovine MUC1 and analogues was observed.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	Bovine Mucin-1 microplate Bovine Mucin-1 standard Bovine Mucin-1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 96 T x 50

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Lieferung vsl. bis 01.01.2026

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