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Bovine Monoamine oxidase A (MAOA) ELISA Kit

Bovine Monoamine oxidase A (MAOA) ELISA Kit

ArtNr	ABK-KTE10330-96Tx50
Hersteller	Abbkine Scientific
Menge	96 T x 50
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Kategorie	Neuroscience Molecular Targets for Neuroscience Cell Biology Cell Status ELISA Oxidative Stress ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Cattle (Bovine)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	MAOA,RP1-201D17__B.2;
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Manufacturer - Applications	This Bovine Monoamine oxidase A (MAOA) ELISA Kit employs a two-site sandwich ELISA to quantitate MAOA in samples. An antibody specific for MAOA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAOA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAOA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAOA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Background	Monoamine oxidase A is an isozyme of monoamine oxidase. It preferentially deaminates norepinephrine (noradrenaline), epinephrine (adrenaline), serotonin, and dopamine (dopamine is equally deaminated by MAO-A and MAO-B). It is inhibited by clorgiline and befloxatone.This gene encodes monoamine oxidase A, an enzyme that degrades amine neurotransmitters, such as dopamine, norepinephrine, and serotonin. The protein localizes to the outer mitochondrial membrane. The gene is adjacent to a related gene (MAOB) on the opposite strand of chromosome X. Mutation in this gene results in monoamine oxidase deficiency, or Brunner syndrome. MAO-A levels in the brain as measured using positron emission tomography are elevated by an average of 34 percent in patients with major depressive disorder.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Bovine Monoamine oxidase A (MAOA) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine MAOA. No significant cross-reactivity or interference between Bovine MAOA and analogues was observed.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	Bovine Monoamine oxidase A microplate Bovine Monoamine oxidase A standard Bovine Monoamine oxidase A detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 96 T x 50

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Lieferung vsl. bis 01.01.2026

Express-Lieferung: 7 Tage sparen

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