Human Von Willebrand factor A domain-containing protein 3A (VWA3A) ELISA Kit

This Human Von Willebrand factor A domain-containing protein 3A (VWA3A) ELISA Kit employs a two-site sandwich ELISA to quantitate VWA3A in samples. An antibody specific for VWA3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVWA3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VWA3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VWA3A bound in the initial step. The color development is stopped and the intensity of the color is measured.

VWA3A

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

This Human Von Willebrand factor A domain-containing protein 3A (VWA3A) ELISA Kit employs a two-site sandwich ELISA to quantitate VWA3A.

Human Von Willebrand factor A domain-containing protein 3A (VWA3A) ELISA Kit has high sensitivity and excellent specificity for detection of Human VWA3A. No significant cross-reactivity or interference between Human VWA3A and analogues was observed.

• Human Von Willebrand factor A domain-containing protein 3A microplate
• Human Von Willebrand factor A domain-containing protein 3A standard
• Human Von Willebrand factor A domain-containing protein 3A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Please inquire

Cell culture supernatants, Serum, Plasma, Other biological fluids

VWA3A, hCG_2039627, FLJ40941, FLJ46765,