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Human Soluble Vascular endothelial cell growth factor receptor 1 (sVEGFR-1) ELISA Kit

Human Soluble Vascular endothelial cell growth factor receptor 1 (sVEGFR-1) ELISA Kit

ArtNr	ABK-KTE60057-48T
Hersteller	Abbkine Scientific
Menge	48 T
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Kategorie	Oncology Neuroscience Neural Signaling Neural Development Neural Adhesion Molecules By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Movement Cell Adhesion Cell Culture Cytokines Adenocarcinoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	VEGFR-1,Flt1,Fms-Related Tyrosine Kinase 1,Vascular Permeability Factor Receptor
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA
Manufacturer - Applications	This Human Soluble Vascular endothelial cell growth factor receptor 1 (VEGFR-1) ELISA Kit employs a two-site sandwich ELISA to quantitate VEGFR-1 in samples. An antibody specific for VEGFR-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVEGFR-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGFR-1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGFR-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	sVEGFR-1
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background	FLT4, is a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA.The FLT4 mRNA signals first became detectable in the angioblasts of head mesenchyme, the cardinal vein, and extraembryonally in the allantois of 8.5-day p.c. postcoitus embryos. In 12.5-day p.c. embryos, the FLT4 signal decorated developing venous and presumptive lymphatic endothelia, but arterial endothelia were negative. FLT4 mRNA became restricted to vascular plexuses devoid of red cells, representing developing lymphatic vessels. In adult human tissues, only the lymphatic endothelia and some high endothelial venules expressed FLT4 mRNA.
Description	Human Soluble Vascular endothelial cell growth factor receptor 1 (sVEGFR-1) ELISA Kit employs a two-site sandwich ELISA to quantitate sVEGFR-1 in samples.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Human Soluble Vascular endothelial cell growth factor receptor 1 (VEGFR-1) ELISA Kit has high sensitivity and excellent specificity for detection of Human VEGFR-1. No significant cross-reactivity or interference between Human VEGFR-1 and analogues was observed.
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	Human Soluble Vascular endothelial cell growth factor receptor 1 microplate Human Soluble Vascular endothelial cell growth factor receptor 1 standard Human Soluble Vascular endothelial cell growth factor receptor 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Limit of detection	15 ng/L
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA
Calibration range	187.5 ng/L-3000 ng/L
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment	ELISA Testing

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 48 T

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Lieferung vsl. bis 04.12.2025

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