Human Target of EGR1 protein 1 (TOE1) ELISA Kit

ELISA

Protein

Gel pack with blue ice.

Target of EGR1 protein 1 is a protein encoded by the TOE1 gene.Egr1, an immediate early transcription factor, responds to diverse stimuli and affects gene transcription to accomplish its biological effects. One important effect of Egr1 expression is to decrease the growth and tumorigenic potential of several tumor cell types. To identify important Egr1 target genes, adapted a methodology involving formaldehyde-induced protein-DNA cross-linking, chromatin immunoprecipitation, and multiplex PCR. Using this approach, report the cloning of a new Egr1 target gene that is able to account, at least in part, for the growth inhibitory activity of Egr1. TOE1 belongs to the CAF1 family. inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. mediates the inhibitory growth effect of EGR1.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

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Sandwich ELISA (quantitative)

Colorimetric

ELISA Testing