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Human Homeobox protein TGIF2LY (TGIF2LY) ELISA Kit

ArtNr ABK-KTE60518-96Tx5
Hersteller Abbkine Scientific
Menge 96 T x 5
Quantity options 48 T 96 T 96 T x 5 96 T x 50
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Human (Homo sapiens)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias TGIF2LY,TGIFLY,TGFB-induced factor 2-like,Y-linked
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA
Manufacturer - Applications
This Human Homeobox protein TGIF2LY (TGIF2LY) ELISA Kit employs a two-site sandwich ELISA to quantitate TGIF2LY in samples. An antibody specific for TGIF2LY has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTGIF2LY present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGIF2LY is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGIF2LY bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category
Protein
Manufacturer - Conjugate / Tag
TGIF2LY
Shipping Temperature
Gel pack with blue ice.
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background
Homeobox protein TGIF2LY (TGIF2LY) is a member of the TALE/TGIF homeobox family of transcription factors. TGIF2LY lies within the male specific region of chromosome Y, in a block of sequence that is thought to be the result of a large X-to-Y transposition. The C-terminus of this protein is divergent from that of its chromosome X homolog (TGIF2LX), suggesting that TGIF2LY may act as a regulator of TGIF2LX.
Description
This Human Homeobox protein TGIF2LY (TGIF2LY) ELISA Kit employs a two-site sandwich ELISA to quantitate TGIF2LY.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Human Homeobox protein TGIF2LY (TGIF2LY) ELISA Kit has high sensitivity and excellent specificity for detection of Human TGIF2LY. No significant cross-reactivity or interference between Human TGIF2LY and analogues was observed.
Usage Notes
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components
• Human Homeobox protein TGIF2LY microplate
• Human Homeobox protein TGIF2LY standard
• Human Homeobox protein TGIF2LY detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Limit of detection
Please inquire
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type
Sandwich ELISA (quantitative)
Calibration range
Please inquire
Detection method
Colorimetric
Sample type
Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment
ELISA Testing
Manufacturer - Search Terms
TGIF2LY, TGIFLY, TGFB-induced factor 2-like, Y-linked

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 T x 5
Lieferbar: In stock
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