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Human Mothers against decapentaplegic homolog 4 (SMAD4) ELISA Kit

Human Mothers against decapentaplegic homolog 4 (SMAD4) ELISA Kit

ArtNr	ABK-KTE60573-96Tx50
Hersteller	Abbkine Scientific
Menge	96 T x 50
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Kategorie	Oncology Neuroscience Targets for Rodent Neurosciences By Cell Type Carcinoma Adenocarcinoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Colorectal Cancer
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	SMAD4,DPC4,JIP,MADH4,MAD,mothers against decapentaplegic homolog 4,OTTHUMP00000163548,SMAD,mothers against DPP homolog 4,deleted in pancreatic carcinoma locus 4,mothers against decapentaplegic homolo
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA
Manufacturer - Applications	This Human Mothers against decapentaplegic homolog 4 (SMAD4) ELISA Kit employs a two-site sandwich ELISA to quantitate SMAD4 in samples. An antibody specific for SMAD4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMAD4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMAD4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMAD4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	SMAD4
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background	SMAD4 is a 552 amino acid protein involved in cell signaling. It belongs to the Darfwin family of proteins which modulate members of the TGF beta protein superfamily. It binds receptor regulated SMADs such as SMAD1 or SMAD2 and forms a complex that binds to DNA and serves as a transcription factor. It is the only known mammalian coSMAD. It is a homolog of the Drosophila protein: "Mothers against decapentaplegic". The SMAD proteins are homologs of both the drosophila protein, mothers against decapentaplegic (MAD) and the C. elegans protein SMA. The name is a combination of the two. During Drosophila research, it was found that a mutation in the gene, MAD, in the mother, repressed the gene, decapentaplegic, in the embryo.
Description	This Human Mothers against decapentaplegic homolog 4 (SMAD4) ELISA Kit employs a two-site sandwich ELISA to quantitate SMAD4.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Human Mothers against decapentaplegic homolog 4 (SMAD4) ELISA Kit has high sensitivity and excellent specificity for detection of Human SMAD4. No significant cross-reactivity or interference between Human SMAD4 and analogues was observed.
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	• Human Mothers against decapentaplegic homolog 4 microplate • Human Mothers against decapentaplegic homolog 4 standard • Human Mothers against decapentaplegic homolog 4 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers
Limit of detection	Please inquire
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Calibration range	Please inquire
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment	ELISA Testing
Manufacturer - Search Terms	SMAD4, DPC4, JIP, MADH4, MAD, mothers against decapentaplegic homolog 4, OTTHUMP00000163548, SMAD, mothers against DPP homolog 4, deleted in pancreatic carcinoma locus 4, mothers against decapentaplegic homolo

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 96 T x 50

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