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Human STE20-like serine/threonine-protein kinase (SLK) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 96Tx5
ArtNr ABK-KTE60579-96Tx5
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Human STE20-like serine/threonine-protein kinase (SLK) ELISA Kit employs a two-site sandwich ELISA to quantitate SLK in samples. An antibody specific for SLK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human STE20-like serine/threonine-protein kinase microplate
Human STE20-like serine/threonine-protein kinase standard
Human STE20-like serine/threonine-protein kinase detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human STE20-like serine/threonine-protein kinase (SLK) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLK. No significant cross-reactivity or interference between Human SLK and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
FYN encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. Fyn is a tyrosine specific phospho-transferase that is a member of the Src family of tyrosine protein kinases.Fyn is primarily localized to the cytoplasmic leaflet of the plasma membrane, where it phosphorylates tyrosine residues on key targets involved in a variety of different signaling pathways. Tyrosine phosphorylation of target proteins by Fyn serves to either regulate target protein activity, and/or to generate a binding site on the target protein that recruits other signaling molecules.
Gene ID
9748
Alternative
SLK; KIAA0204; LOSK; MGC133067; STK2; bA16H23.1; se20-9; CTCL tumor antigen se20-9; Long Ste20-like Kinase; SNF1 (sucrose nonfermenting; yeast; homolog)-like kinase; SNF1 sucrose nonfermenting like kinase;
Accession
Q9H2G2

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96Tx5
Lieferbar: In stock
lieferbar

Lieferung vsl. bis 13.06.2024 

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