Human NAD-dependent deacetylase sirtuin-6 (SIRT6/SIR2L6) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Human NAD-dependent deacetylase sirtuin-6 microplate
Human NAD-dependent deacetylase sirtuin-6 standard
Human NAD-dependent deacetylase sirtuin-6 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

SIRT6 encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.Sirtuins can be divided into 4 main classes in eukaryotes based on amino acid sequence motifs, with yeast Sir2 belonging to class Ia. The deduced 355-amino acid SIRT6 protein is a class IV sirtuin, a class not present in prokaryotes.

SIRT6; SIR2L6; sir2-related protein type 6; sirtuin 6; sirtuin type 6