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Human Secreted frizzled-related protein 4 (SFRP4) ELISA Kit

Human Secreted frizzled-related protein 4 (SFRP4) ELISA Kit

ArtNr	ABK-KTE60680-96T
Hersteller	Abbkine Scientific
Menge	96 T
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Kategorie	Oncology By Cell Type Blastoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	SFRP4,FRP-4,FRPHE,MGC26498,secreted frizzled-related protein 4,secreted frizzled-related protein 4,secreted frizzled-related protein 4
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA
Manufacturer - Applications	This Human Secreted frizzled-related protein 4 (SFRP4) ELISA Kit employs a two-site sandwich ELISA to quantitate SFRP4 in samples. An antibody specific for SFRP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySFRP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFRP4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFRP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	SFRP4
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background	Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. Sequence analysis predicted that the 346-amino acid SFRP4 protein contains an N-terminal signal peptide, no transmembrane domain, and a hydrophilic C terminus. In situ hybridization analysis demonstrated exclusive expression in stromal and myometrial cells, particularly in endometrium and breast. Upregulated expression was detected in endometrial and breast carcinomas.
Description	This Human Secreted frizzled-related protein 4 (SFRP4) ELISA Kit employs a two-site sandwich ELISA to quantitate SFRP4.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Human Secreted frizzled-related protein 4 (SFRP4) ELISA Kit has high sensitivity and excellent specificity for detection of Human SFRP4. No significant cross-reactivity or interference between Human SFRP4 and analogues was observed.
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	• Human Secreted frizzled-related protein 4 microplate • Human Secreted frizzled-related protein 4 standard • Human Secreted frizzled-related protein 4 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers
Limit of detection	Please inquire
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Calibration range	Please inquire
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment	ELISA Testing
Manufacturer - Search Terms	SFRP4, FRP-4, FRPHE, MGC26498, secreted frizzled-related protein 4, secreted frizzled-related protein 4, secreted frizzled-related protein 4

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 96 T

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Lieferung vsl. bis 01.01.2026

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