Vergleich

Human Serum deprivation response (SDPR) ELISA Kit

ArtNr ABK-KTE60724-48T
Hersteller Abbkine Scientific
Menge 48 T
Quantity options 48 T 96 T 96 T x 5 96 T x 50
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Human (Homo sapiens)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias SDPR,PS-p68,SDR,cavin-2,phosphatidylserine binding protein,serum deprivation response (phosphatidylserine-binding protein),serum deprivation response protein
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA
Manufacturer - Applications
This Human Serum deprivation response (SDPR) ELISA Kit employs a two-site sandwich ELISA to quantitate SDPR in samples. An antibody specific for SDPR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySDPR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDPR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDPR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category
Protein
Manufacturer - Conjugate / Tag
SDPR
Shipping Temperature
Gel pack with blue ice.
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background
SDPR encodes a calcium-independent phospholipid-binding protein whose expression increases in serum-starved cells. This protein has also been shown to be a substrate for protein kinase C (PKC) phosphorylation.
The SDPR cDNA encodes a deduced 425-amino acid peptide with a calculated molecular mass of 47.2 kD. The human and mouse proteins share 84% sequence identity; both contain a leucine zipper-like domain with 7 repeats and 2 putative protein kinase C phosphorylation sites. Northern blot analysis of various human tissues showed nearly ubiquitous expression of a 3.1-kb transcript, which was always coexpressed with a shorter transcript. Highest expression was detected in heart and lung.SDPR is able to bind PS liposomes in a calcium-independent manner.
Description
This Human Serum deprivation response (SDPR) ELISA Kit employs a two-site sandwich ELISA to quantitate SDPR.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Human Serum deprivation response (SDPR) ELISA Kit has high sensitivity and excellent specificity for detection of Human SDPR. No significant cross-reactivity or interference between Human SDPR and analogues was observed.
Usage Notes
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components
• Human Serum deprivation response microplate
• Human Serum deprivation response standard
• Human Serum deprivation response detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Limit of detection
Please inquire
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type
Sandwich ELISA (quantitative)
Calibration range
Please inquire
Detection method
Colorimetric
Sample type
Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment
ELISA Testing
Manufacturer - Search Terms
SDPR, PS-p68, SDR, cavin-2, phosphatidylserine binding protein, serum deprivation response (phosphatidylserine-binding protein), serum deprivation response protein

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 48 T
Lieferbar: In stock
lieferbar

Vergleichen

Auf den Wunschzettel

Angebot anfordern

Lieferzeit anfragen

Technische Frage stellen

Bulk-Anfrage stellen

Fragen zum Produkt?
 
Schließen