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Human Rho-related GTP-binding protein RhoC (RHOC) ELISA Kit

Human Rho-related GTP-binding protein RhoC (RHOC) ELISA Kit

ArtNr	ABK-KTE60796-96Tx5
Hersteller	Abbkine Scientific
Menge	96 T x 5
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Kategorie	Cell Biology Cytoskeleton ELISA ELISA & Immunoassays ELISA Kits Immunoassay small GTPases Rho Subfamily
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	RHOC,RP11-426L16.4,ARH9,ARHC,H9,MGC1448,MGC61427,RHOH9,OTTHUMP00000013802,OTTHUMP00000013805,OTTHUMP00000013807,RAS-related homolog 9,Rho-related GTP-binding protein RhoC,oncogene RHO H9,rhoC GTPa
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA
Manufacturer - Applications	This Human Rho-related GTP-binding protein RhC (RHOC) ELISA Kit employs a two-site sandwich ELISA to quantitate RHOC in samples. An antibody specific for RHOC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRHOC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RHOC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RHOC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	RHOC
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background	RhoC (Ras homolog gene family, member C) is a small (~21 kDa) signaling G protein (more specifically a GTPase), and is a member of the Rac subfamily of the family Rho family of GTPases.It cycles between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. It is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Description	This Human Rho-related GTP-binding protein RhoC (RHOC) ELISA Kit employs a two-site sandwich ELISA to quantitate RHOC.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Human Rho-related GTP-binding protein RhoC (RHOC) ELISA Kit has high sensitivity and excellent specificity for detection of Human RHOC. No significant cross-reactivity or interference between Human RHOC and analogues was observed.
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	• Human Rho-related GTP-binding protein RhoC microplate • Human Rho-related GTP-binding protein RhoC standard • Human Rho-related GTP-binding protein RhoC detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers
Limit of detection	Please inquire
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Calibration range	Please inquire
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment	ELISA Testing
Manufacturer - Search Terms	RHOC, RP11-426L16.4, ARH9, ARHC, H9, MGC1448, MGC61427, RHOH9, OTTHUMP00000013802, OTTHUMP00000013805, OTTHUMP00000013807, RAS-related homolog 9, Rho-related GTP-binding protein RhoC, oncogene RHO H9, rhoC GTPa

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 96 T x 5

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