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Human Alpha-fetoprotein Lens culinaris agglutiin 3 (AFP-L3) ELISA Kit

ArtNr ABK-KTE60958-96Tx5
Hersteller Abbkine Scientific
Menge 96 T x 5
Quantity options 48 T 96 T 96 T x 5 96 T x 50
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Human (Homo sapiens)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias AFP-L3
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA
Manufacturer - Applications
This Human Alpha-fetoprotein Lens culinaris agglutiin 3 (AFP-L3) ELISA Kit employs a two-site sandwich ELISA to quantitate AFP-L3 in samples. An antibody specific for AFP-L3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAFP-L3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AFP-L3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AFP-L3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category
Protein
Manufacturer - Conjugate / Tag
AFP-L3
Shipping Temperature
Gel pack with blue ice.
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background
AFP-L3 is one of AFP fractions which is synthesized by malignant cell and comprise special oligosaccharide.It is a new generation of tumor marker for HCC and yields useful information on HCC for clinical decision making. Reports at home or abroad have showed that if the ratio of AFP-L3 increased over 10%, the risk of catching HCC had increased highly, and non-malignant diseases hardly synthesize this AFP fraction(AFP-L3).AFP-L3 can be detected 9-12 months earlier and shows the remarkable precaution effect to HCC. AFP-L3 is the fucosylated variant of AFP produced specifically by HCC. AFP-L3 bears strong affinity to the sugar chain of Lens culinaris, a type of lentil. AFP-L3 is less sensitive but more specific than AFP, and therefore cannot replace AFP. The value of AFP-L3 lies mainly in distinguishing HCC from other conditions when AFP is elevated.
Description
This Human Alpha-fetoprotein Lens culinaris agglutiin 3 (AFP-L3) ELISA Kit employs a two-site sandwich ELISA to quantitate AFP-L3.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Human Alpha-fetoprotein Lens culinaris agglutiin 3 (AFP-L3) ELISA Kit has high sensitivity and excellent specificity for detection of Human AFP-L3. No significant cross-reactivity or interference between Human AFP-L3 and analogues was observed.
Usage Notes
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components
• Human Alpha-fetoprotein Lens culinaris agglutiin 3 microplate
• Human Alpha-fetoprotein Lens culinaris agglutiin 3 standard
• Human Alpha-fetoprotein Lens culinaris agglutiin 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Limit of detection
Please inquire
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type
Sandwich ELISA (quantitative)
Calibration range
Please inquire
Detection method
Colorimetric
Sample type
Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment
ELISA Testing
Manufacturer - Search Terms
AFP-L3

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 T x 5
Lieferbar: In stock
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