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Human CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) ELISA Kit

Human CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) ELISA Kit

ArtNr	ABK-KTE61155-96Tx5
Hersteller	Abbkine Scientific
Menge	96 T x 5
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Kategorie	Oncology Neuroscience Metabolism Diabetes Targets for Rodent Neurosciences Neural Signaling By Cell Type Carcinoma Melanoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	PRKACA,MGC102831,MGC48865,PKACA,PKA C-alpha,cAMP-dependent protein kinase catalytic subunit alpha,cAMP-dependent protein kinase catalytic subunit alpha,isoform 1,protein kinase A catalytic subunit
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA
Manufacturer - Applications	This Human CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKACA in samples. An antibody specific for PRKACA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKACA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKACA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKACA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	PRKACA
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background	cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the AMP-activated protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PRKACais a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Description	This Human CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKACA.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Human CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRKACA. No significant cross-reactivity or interference between Human PRKACA and analogues was observed.
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	• Human CAMP-dependent protein kinase catalytic subunit alpha microplate • Human CAMP-dependent protein kinase catalytic subunit alpha standard • Human CAMP-dependent protein kinase catalytic subunit alpha detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers
Limit of detection	Please inquire
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Calibration range	Please inquire
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment	ELISA Testing
Manufacturer - Search Terms	PRKACA, MGC102831, MGC48865, PKACA, PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha, cAMP-dependent protein kinase catalytic subunit alpha, isoform 1, protein kinase A catalytic subunit

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 96 T x 5

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