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Human Peroxisome proliferator-activated receptor gamma coactivator 1-beta (PPARGC1B) ELISA Kit

Human Peroxisome proliferator-activated receptor gamma coactivator 1-beta (PPARGC1B) ELISA Kit

ArtNr	ABK-KTE61194-96T
Hersteller	Abbkine Scientific
Menge	96 T
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Kategorie	Oncology Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism By Cell Type Carcinoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Thyroid Cancer Other Lipid\Cholesterol Metabolism Modulators
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	PPARGC1B,ERRL1,PERC,PGC-1(beta),PGC1B,PGC-1-related estrogen receptor alpha coactivator,peroxisome proliferative activated receptor,gamma,coactivator 1,beta
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA
Manufacturer - Applications	This Human Peroxisome proliferator-activated receptor gamma coactivator 1-beta (PPARGC1B) ELISA Kit employs a two-site sandwich ELISA to quantitate PPARGC1B in samples. An antibody specific for PPARGC1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPARGC1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPARGC1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPARGC1B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	PPARGC1B
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background	PPARgC1b stimulates the activity of several transcription factors and nuclear receptors, including estrogen receptor alpha, nuclear respiratory factor 1, and glucocorticoid receptor. The encoded protein may be involved in fat oxidation, non-oxidative glucose metabolism, and the regulation of energy expenditure. This protein is downregulated in prediabetic and type 2 diabetes mellitus patients. Certain allelic variations in this gene increase the risk of the development of obesity. Ppargc1b encodes a predicted 1, 014-amino acid protein, and human and mouse PPARGC1B share 70% amino acid sequence identity. Ppargc1b contains 3 N-terminal LXXLL motifs, 2 glutamic/aspartic acid-rich acidic domains, a binding site for host cell factor (HCF1), and a C-terminal RNA recognition motif.
Description	Human Peroxisome proliferator-activated receptor gamma coactivator 1-beta (PPARGC1B) ELISA Kit employs a two-site sandwich ELISA to quantitate PPARGC1B in samples.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Human Peroxisome proliferator-activated receptor gamma coactivator 1-beta (PPARGC1B) ELISA Kit has high sensitivity and excellent specificity for detection of Human PPARGC1B. No significant cross-reactivity or interference between Human PPARGC1B and analogues was observed.
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	Human Peroxisome proliferator-activated receptor gamma coactivator 1-beta microplate Human Peroxisome proliferator-activated receptor gamma coactivator 1-beta standard Human Peroxisome proliferator-activated receptor gamma coactivator 1-beta detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Limit of detection	1.0 pg/mL
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA
Calibration range	30 pg/mL-480 pg/mL
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment	ELISA Testing

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 96 T

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Lieferung vsl. bis 04.12.2025

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