Human 6-phosphofructokinase, muscle type (PFKM) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Human 6-phosphofructokinase, muscle type microplate
Human 6-phosphofructokinase, muscle type standard
Human 6-phosphofructokinase, muscle type detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Phosphofructokinase-1 (PFK-1) is the most important regulatory enzyme (EC 2.7.1.11) of glycolysis. It is an allosteric enzyme made of 4 subunits and controlled by several activators and inhibitors. PFK-1 catalyzes one of the important "committed" steps of glycolysis, the conversion of fructose 6-phosphate and ATP to fructose 1, 6-bisphosphate and ADP.
PFK1 is allosterically inhibited by ATP and citrate (from the citric acid cycle) and its product. It is also inhibited by low pH to prevent the accumulation of hydrogen ions in muscle. The enzyme has two sites with different affinities for ATP which is both a substrate and an inhibitor.PFK1 is allosterically activated by a high concentration of AMP, but the most potent activator is fructose 2, 6-bisphosphate, which is also produced from fructose-6-phosphate by PFK2.

PFKM; GSD7; MGC8699; PFK-1; PFK1; PFKA; PFKX; 6-phosphofructo-1-kinase; phosphofructo-1-kinase isozyme A; phosphofructokinase; polypeptide X; phosphohexokinase