Human Programmed cell death 4 (PDCD4) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Human Programmed cell death 4 microplate
Human Programmed cell death 4 standard
Human Programmed cell death 4 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

PDCD4 encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified.The deduced 458-amino acid protein has a calculated molecular mass of 50.6 kD. PDCD4 is an acidic protein with characteristics of a nuclear nonhistone protein. It contains 2 overlapping GxGxxG nucleotide-binding motifs, which are characteristic of protein kinases, but it does not have a kinase catalytic domain. PDCD4 also has several putative serine and threonine phosphorylation sites. Northern blot analysis detected transcripts of about 2.7 and 3.8 kb in HeLa cell total RNA.

PDCD4; RP11-348N5.4; H731; MGC33046; MGC33047; nuclear antigen H731; programmed cell death 4