Human Carboxyterminal propeptide of type I procollagen (PICP) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Human Carboxyterminal propeptide of type I procollagen microplate
Human Carboxyterminal propeptide of type I procollagen standard
Human Carboxyterminal propeptide of type I procollagen detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

The carboxyterminal propeptide of type I procollagen (PICP) and the aminoterminal propeptide of type I procollagen (PINP) are enzymatically removed from the procollagen molecule by specific proteases.
PICP and PINP are indicators of the synthesis of type I collagen. Cleavage of PICP is required for the initiation of fibril formation, whereas PINP may be retained in the collagen molecule resulting in type I pN-collagen. The retention of PINP is transient, unlike the presence of IIIpN-collagen, and has been considered to regulate fibril diameter (Fleischmajer et al. 1985).
PICP is a trimeric, globular protein consisting of three polypeptide chains: two proalpha1(I) and one proalpha2(I) chains. The molecular mass of PICP is 100 000 and it is cleared by mannose receptors in liver endothelial cells (Smedsrød et al. 1990).