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Human Peptide chain release factor 1-like, mitochondrial (MTRF1L) ELISA Kit

Human Peptide chain release factor 1-like, mitochondrial (MTRF1L) ELISA Kit

ArtNr	ABK-KTE61476-48T
Hersteller	Abbkine Scientific
Menge	48 T
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Kategorie	ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	MTRF1L,RP1-101K10.1,MGC102748,MRF1L,mtRF1a,mitochondrial release factor 1 like
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA
Manufacturer - Applications	This Human Peptide chain release factor 1-like, mitochondrial (MTRF1L) ELISA Kit employs a two-site sandwich ELISA to quantitate MTRF1L in samples. An antibody specific for MTRF1L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTRF1L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTRF1L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTRF1L bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	MTRF1L
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background	Mitochondrial translational release factor 1-like encoded by MTRF1L plays a role in mitochondrial translation termination, and is thought to be a release factor that is involved in the dissociation of the complete protein from the final tRNA, the ribosome, and the cognate mRNA. Mitochondrial translational release factor 1-like acts upon UAA and UAG stop codons, but has no in vitro activity against UGA, which encodes tryptophan in human mitochondrion, or, the mitochondrial non-cognate stop codons, AGA and AGG. Mitochondrial translational release factor 1-like shares sequence similarity to bacterial release factors. Pseudogenes of this gene are found on chromosomes 4, 8, and 11. Alternative splicing results in multiple transcript variants.
Description	This Human Peptide chain release factor 1-like, mitochondrial (MTRF1L) ELISA Kit employs a two-site sandwich ELISA to quantitate MTRF1L.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Human Peptide chain release factor 1-like, mitochondrial (MTRF1L) ELISA Kit has high sensitivity and excellent specificity for detection of Human MTRF1L. No significant cross-reactivity or interference between Human MTRF1L and analogues was observed.
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	• Human Peptide chain release factor 1-like, mitochondrial microplate • Human Peptide chain release factor 1-like, mitochondrial standard • Human Peptide chain release factor 1-like, mitochondrial detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers
Limit of detection	Please inquire
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Calibration range	Please inquire
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment	ELISA Testing
Manufacturer - Search Terms	MTRF1L, RP1-101K10.1, MGC102748, MRF1L, mtRF1a, mitochondrial release factor 1 like

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 48 T

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