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Human Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) ELISA Kit

ArtNr ABK-KTE61731-48T
Hersteller Abbkine Scientific
Menge 48 T
Quantity options 48 T 96 T 96 T x 5 96 T x 50
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Human (Homo sapiens)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias MAPKAP1,RP11-269P11.1,JC310,MGC2745,MIP1,SIN1,SIN1b,SIN1g,MEKK2-interacting protein 1,OTTHUMP00000022141,SAPK-interacting protein 1,ras inhibitor MGC2745,stress-activated map kinase interacting prote
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA
Manufacturer - Applications
This Human Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPKAP1 in samples. An antibody specific for MAPKAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPKAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPKAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPKAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category
Protein
Manufacturer - Conjugate / Tag
MAPKAP1
Shipping Temperature
Gel pack with blue ice.
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background
Target of rapamycin complex 2 subunit MAPKAP1 is a protein similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene.
The deduced 522-amino acid protein has a nuclear localization signal, 2 bipartite nuclear localization signals, a peroxisomal targeting signal, and a PEST motif for rapid protein degradation. Comparison of SIN1 with homologs from various species revealed a short, highly conserved region, designated Box1, located within a larger conserved domain, designated CRIM (conserved region in middle).
Description
This Human Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPKAP1.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Human Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAPKAP1. No significant cross-reactivity or interference between Human MAPKAP1 and analogues was observed.
Usage Notes
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components
• Human Target of rapamycin complex 2 subunit MAPKAP1 microplate
• Human Target of rapamycin complex 2 subunit MAPKAP1 standard
• Human Target of rapamycin complex 2 subunit MAPKAP1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Limit of detection
Please inquire
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type
Sandwich ELISA (quantitative)
Calibration range
Please inquire
Detection method
Colorimetric
Sample type
Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment
ELISA Testing
Manufacturer - Search Terms
MAPKAP1, RP11-269P11.1, JC310, MGC2745, MIP1, SIN1, SIN1b, SIN1g, MEKK2-interacting protein 1, OTTHUMP00000022141, SAPK-interacting protein 1, ras inhibitor MGC2745, stress-activated map kinase interacting prote

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 48 T
Lieferbar: In stock
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