Human Collagen Type I Alpha 1 (COL1A1) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 96Tx50
ArtNr ABK-KTE62512-96Tx50
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Human Collagen Type I Alpha 1 (COL1A1) ELISA Kit employs a two-site sandwich ELISA to quantitate COL1A1 in samples. An antibody specific for COL1A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCOL1A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COL1A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COL1A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human Collagen Type I Alpha 1 microplate
Human Collagen Type I Alpha 1 standard
Human Collagen Type I Alpha 1 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human Collagen Type I Alpha 1 (COL1A1) ELISA Kit has high sensitivity and excellent specificity for detection of Human COL1A1. No significant cross-reactivity or interference between Human COL1A1 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Collagen, type I, alpha 1 is the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage.The COL1A1 gene produces a component of type I collagen, called the pro-alpha1(I) chain. This chain combines with another pro-alpha1(I) chain and also with a pro-alpha2(I) chain (produced by the COL1A2 gene) to make a molecule of type I procollagen. These triple-stranded, rope-like procollagen molecules must be processed by enzymes outside the cell. Once these molecules are processed, they arrange themselves into long, thin fibrils that cross-link to one another in the spaces around cells. The cross-links result in the formation of very strong mature type I collagen fibers.
Alternative
COL1A1; COL-1A1; COL1-A1; COL1A-1; OI4; Collagen Alpha-1(I)chain
Menge: 96Tx50
Lieferbar: In stock
Listenpreis: 17.954,99 €
Preis: 17.954,99 €
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