Human CC-Chemokine Receptor 5 (CCR5) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 96Tx50
ArtNr ABK-KTE62546-96Tx50
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Human CC-Chemokine Receptor 5 (CCR5) ELISA Kit employs a two-site sandwich ELISA to quantitate CCR5 in samples. An antibody specific for CCR5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCCR5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCR5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCR5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human CC-Chemokine Receptor 5 microplate
Human CC-Chemokine Receptor 5 standard
Human CC-Chemokine Receptor 5 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human CC-Chemokine Receptor 5 (CCR5) ELISA Kit has high sensitivity and excellent specificity for detection of Human CCR5. No significant cross-reactivity or interference between Human CCR5 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
CCR5 is a member of the beta chemokine receptors family of integral membrane proteins.In Humans, the CCR5 gene location is on the short (p) arm at position 21 on chromosome 3. Certain populations have inherited the Delta 32 mutation resulting in the Genetic deletion of the CCR5 gene. Homozygous carriers of this mutation are resistant to HIV-1 infection. The CCR5 protein is a seven transmembrane protein which functions as a chemokine receptor in the CC chemokine group. The natural chemokine ligands that bind to this receptor are RANTES, MIP-1alpha and MIP-1beta. CCR5 is predominantly expressed on T cells, macrophages, dendritic cells and microglia. It is likely that CCR5 plays a role in inflammatory responses to infection, though its exact role in normal immune function is unclear.
Gene ID
497017
Alternative
CCR5; CC-CKR-5; CCCKR5; CD195; CKR-5; CKR5; CMKBR5; FLJ78003; IDDM22; C-C chemokine receptor type 5; C-C motif chemokine receptor 5 A159A; OTTHUMP00000209784; chemokine receptor CCR5; chemr13
Accession
Q2HJ17

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Menge: 96Tx50
Lieferbar: In stock
Listenpreis: 21.545,99 €
Preis: 21.545,99 €
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