Human Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

Hersteller Abbkine Scientific
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 96Tx50
ArtNr ABK-KTE62572-96Tx50
eClass 6.1 32160605
eClass 9.0 32160605
Applications notes
This Human Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit employs a two-site sandwich ELISA to quantitate ALCAM in samples. An antibody specific for ALCAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyALCAM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALCAM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALCAM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human Activated Leukocyte Cell Adhesion Molecule microplate
Human Activated Leukocyte Cell Adhesion Molecule standard
Human Activated Leukocyte Cell Adhesion Molecule detect antibody
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit has high sensitivity and excellent specificity for detection of Human ALCAM. No significant cross-reactivity or interference between Human ALCAM and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
ALCAM/CD166 is a member of the immunoglobulin gene superfamily belonging to a subgroup with five extracellular immunoglobulin-like domains(VVC2C2C2), which includes MCAM/CD146/MUC18 and B-CAM/Lutheran. These molecules are involved in the development and maintenance of tissue architecture, neurogenesis, hematopoiesis, immune responses and tumor progression. ALCAM/CD166 mediates cell-cell clustering through homophilic (ALCAM-ALCAM) and heterophilic (ALCAM-CD6) interactions. Its pattern of expression in human tissues and cells is broad and includes epithelia, neurons, lymphoid and myeloid cells, hematopoietic and mesenchymal stem cells. The relevance of the engagement of the heterophilic pair CD166-CD6 for optimal activation of T cells has been recently demonstrated.
Gene ID
ALCAM; CD166; FLJ38514; MEMD; MGC71733; activated leucocyte cell adhesion molecule

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Menge: 96Tx50
Lieferbar: In stock
Listenpreis: 20.526,53 €
Preis: 20.526,53 €


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