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Human Epidermal growth factor receptor (EGFR) ELISA Kit

ArtNr ABK-KTE62577-48T
Hersteller Abbkine Scientific
Menge 48 T
Quantity options 48 T 96 T 96 T x 5 96 T x 50
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Human (Homo sapiens)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias EGFR,ERBB,ERBB1,HER1,PIG61,mENA,avian erythroblastic leukemia viral (v-erb-b) oncogene homolog,cell growth inhibiting protein 40,cell proliferation-inducing protein 61,epidermal growth factor recept
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA
Manufacturer - Applications
This Human Epidermal growth factor receptor (EGFR) ELISA Kit employs a two-site sandwich ELISA to quantitate EGFR in samples. An antibody specific for EGFR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEGFR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGFR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGFR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category
Protein
Manufacturer - Conjugate / Tag
EGFR
Shipping Temperature
Gel pack with blue ice.
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background
The epidermal growth factor receptor is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer. EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα). ErbB2 has no known direct activating ligand, and may be in an activated state constitutively or become active upon heterodimerization with other family members such as EGFR.
Description
This Human Epidermal growth factor receptor (EGFR) ELISA Kit employs a two-site sandwich ELISA to quantitate EGFR.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Human Epidermal growth factor receptor (EGFR) ELISA Kit has high sensitivity and excellent specificity for detection of Human EGFR. No significant cross-reactivity or interference between Human EGFR and analogues was observed.
Usage Notes
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components
• Human Epidermal growth factor receptor microplate
• Human Epidermal growth factor receptor standard
• Human Epidermal growth factor receptor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Limit of detection
Please inquire
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type
Sandwich ELISA (quantitative)
Calibration range
Please inquire
Detection method
Colorimetric
Sample type
Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment
ELISA Testing
Manufacturer - Search Terms
EGFR, ERBB, ERBB1, HER1, PIG61, mENA, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, epidermal growth factor recept

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 48 T
Lieferbar: In stock
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