Human R-type pyruvate kinase (R-PK) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 96Tx50
ArtNr ABK-KTE62579-96Tx50
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Human R-type pyruvate kinase (R-PK) ELISA Kit employs a two-site sandwich ELISA to quantitate PKLR in samples. An antibody specific for PKLR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPKLR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PKLR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PKLR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human R-type pyruvate kinase microplate
Human R-type pyruvate kinase standard
Human R-type pyruvate kinase detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human R-type pyruvate kinase (R-PK) ELISA Kit has high sensitivity and excellent specificity for detection of Human PKLR. No significant cross-reactivity or interference between Human PKLR and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
The protein encoded by R-PK is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Alternatively spliced transcript variants encoding distinct isoforms have been described.
Gene ID
5313
Alternative
PKLR; PK1; PKL; PKR; PKRL; RPK; R-type/L-type pyruvate kinase; pyruvate kinase 1; pyruvate kinase isozyme R/L; pyruvate kinase type L; pyruvate kinase; liver and blood cell; red cell/liver pyruvate kinase
Accession
P30613
Menge: 96Tx50
Lieferbar: In stock
Listenpreis: 17.954,99 €
Preis: 17.954,99 €
lieferbar

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