Manufacturer |
Abbkine Scientific
|
Category |
|
Type |
Elisa-Kit |
Specific against |
Human |
Applications |
ELISA |
Amount |
96Tx50 |
Item no. |
ABK-KTE62579-96Tx50 |
eClass 6.1 |
32160605 |
eClass 9.0 |
32160605 |
Available |
|
Applications notes |
This Human R-type pyruvate kinase (R-PK) ELISA Kit employs a two-site sandwich ELISA to quantitate PKLR in samples. An antibody specific for PKLR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPKLR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PKLR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PKLR bound in the initial step. The color development is stopped and the intensity of the color is measured. |
Detection method |
Colorimetric |
Sample type |
Cell culture supernatants#Serum#Plasma#Other biological fluids |
Assay type |
Sandwich ELISA (quantitative) |
Assay duration |
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
Kit components |
Human R-type pyruvate kinase microplate Human R-type pyruvate kinase standard Human R-type pyruvate kinase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers |
Features & Benefits |
Human R-type pyruvate kinase (R-PK) ELISA Kit has high sensitivity and excellent specificity for detection of Human PKLR. No significant cross-reactivity or interference between Human PKLR and analogues was observed. |
Usage notes |
Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate. |
Storage instructions |
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols. |
Shipping |
Gel pack with blue ice. |
Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background |
The protein encoded by R-PK is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Alternatively spliced transcript variants encoding distinct isoforms have been described. |
Gene ID |
5313 |
Alternative |
PKLR; PK1; PKL; PKR; PKRL; RPK; R-type/L-type pyruvate kinase; pyruvate kinase 1; pyruvate kinase isozyme R/L; pyruvate kinase type L; pyruvate kinase; liver and blood cell; red cell/liver pyruvate kinase |
Accession |
P30613 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.