« Zurück
Startseite /
Mouse Tubulin polymerization-promoting protein (TPPP) ELISA Kit

Mouse Tubulin polymerization-promoting protein (TPPP) ELISA Kit

ArtNr	ABK-KTE70063-96T
Hersteller	Abbkine Scientific
Menge	96 T
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Kategorie	Cell Biology Cytoskeleton ELISA ELISA & Immunoassays ELISA Kits Immunoassay Mikrotubules
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Mouse (Murine, Mus musculus)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	TPPP,TPPP/p25,TPPP1,p24,p25,p25alpha,brain specific protein p25 alpha,glycogen synthase kinase 3 (GSK3) inhibitor p24,tubulin polymerization-promoting protein
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA
Manufacturer - Applications	This Mouse Tubulin polymerization-promoting protein (TPPP) ELISA Kit employs a two-site sandwich ELISA to quantitate TPPP in samples. An antibody specific for TPPP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPPP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPPP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPPP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	TPPP
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background	The novel basic, heat-stable tubulin polymerization promoting protein TPPP/p25 is associated with microtubules in vitro and can induce the formation of aberrant microtubule assemblies. Immunohistochemistry and confocal microscopy demonstrates that TPPP/p25 is enriched in filamentous alpha-synuclein bearing Lewy bodies of Parkinson's (PD) and diffuse Lewy body disease (DLBD), as well as glial inclusions of multiple system atrophy (MSA). There is a correlation between TPPP/p25 and alpha-synuclein immunoreactivity in Western blot. In contrast, TPPP/p25 is not associated with abnormally phosphorylated tau in various inclusions of Pick's disease (PiD), progressive supranuclear palsy (PSP), and corticobasal degeneration (CBD).
Description	This Mouse Tubulin polymerization-promoting protein (TPPP) ELISA Kit employs a two-site sandwich ELISA to quantitate TPPP.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Mouse Tubulin polymerization-promoting protein (TPPP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TPPP. No significant cross-reactivity or interference between Mouse TPPP and analogues was observed.
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	• Mouse Tubulin polymerization-promoting protein microplate • Mouse Tubulin polymerization-promoting protein standard • Mouse Tubulin polymerization-promoting protein detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers
Limit of detection	Please inquire
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Calibration range	Please inquire
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment	ELISA Testing
Manufacturer - Search Terms	TPPP, TPPP/p25, TPPP1, p24, p25, p25alpha, brain specific protein p25 alpha, glycogen synthase kinase 3 (GSK3) inhibitor p24, tubulin polymerization-promoting protein

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Arbeitsanleitung (Data Sheet) anfordern

Produkt-Datenblatt anfordern

Sicherheitsdatenblatt anfordern

Einfache Bestellung Periodische Bestellung

Menge: 96 T

lieferbar

Lieferung vsl. bis 18.12.2025

Express-Lieferung: 10 Tage sparen

Vergleichen

Auf den Wunschzettel

Angebot anfordern